Table 1.

In vitro kinetic parameters of the CYP71A10 enzyme for four phenylurea substrates

Substrate	Km,app, μM	Vmax, pmol/min per mg of protein	Turnover, min−1
Fluometuron	14.9  (1.0)*	303.6  (10.8)	6.8  (0.24)
Linuron	9.8  (2.1)	125.6  (12.0)	2.8  (0.27)
Chlortoluron	1.0  (0.2)	29.4  (2.2)	0.7  (0.05)
Diuron	1.5  (0.3)	16.8  (1.6)	0.4  (0.04)

Assays were repeated three times for linuron and twice for all other substrates. Concentration ranges used for fluometuron, linuron, chlortoluron, and diuron were 3.2–27.7, 3.8–28.3, 0.7–4.0, and 0.7–3.7 μM, respectively. 

^*Values in parentheses represent standard error.