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. 2003 Jun;47(6):1912–1921. doi: 10.1128/AAC.47.6.1912-1921.2003

FIG. 5.

FIG. 5.

Pretreatment with IFN and ribavirin enhances anti-CD95-induced cleavage of PARP. HepG2 cells were pretreated for 24 h with medium (lanes 1 to 3), ribavirin (lanes 4 and 5), IFN (lanes 6 and 7), or the combination of ribavirin and IFN (lanes 8 and 9). Subsequently the cells were stimulated for 4 h with medium (lane 3), 0.25 μg of anti-CD95 per ml (lanes 1, 2, and 4 to 9), or anti-CD95 plus CHX (lane 1) as a positive control. Cell lysates were then assayed for PARP by immunoblot analysis. Uncleaved PARP is indicated by the open arrowhead, and cleavage products are indicated by the solid closed arrowhead. Note that the nonspecific band above 115 kDa serves as the loading control.