TABLE 4.
Four-week administration of a combination of FTC (100 mg/kg) and l-FMAU (40 mg/kg) in experimentally infected ducklings
| Group and duck no. | Viremia (pg/ml)a
|
Viral productionb (AUC) (day 3-31) | Intrahepaticc viral DNA (day 223) | ||
|---|---|---|---|---|---|
| Day 10 | Day 31 | Day 223 | |||
| Controls | |||||
| 321 | 4.7 | 4.5 | 3.3 | 5.2 | 339 |
| 322 | 4.3 | 3.4 | 4.9 | ||
| 323 | 4.2 | 4.2 | 3.1 | 5.0 | 80 |
| 324 | 4.2 | 4.1 | 3.7 | 4.8 | 1,894 |
| 325 | 4.2 | 3.1 | 3.5 | 4.9 | 1,693 |
| 326 | 4.2 | 2.7 | 0.1 | 4.8 | 0.14 |
| 327 | 3.7 | 1.1 | 4.5 | ||
| 328 | 4.1 | 3.5 | 3.7 | 4.7 | 345 |
| 329 | 4.1 | 3.1 | 4.5 | ||
| 330 | 4.2 | 3.6 | 3.2 | 4.8 | 196 |
| Mean | 4.2 ± 0.3 | 3.4 ± 1 | 2.9 ± 1.3 | 4.8 ± 0.2 | 649.5 ± 793.5 |
| l-FMAU + FTC | |||||
| 331 | 2.1 | 0 | 2.7 | ||
| 332 | 3.7 | 2.9 | 3.2 | 4.3 | 127 |
| 333 | 1.6 | ||||
| 334 | 3.6 | 2.9 | 4.7 | ||
| 335 | 3.5 | 3.4 | 3.4 | 4.4 | 160 |
| 336 | 2.9 | 0.3 | 3.1 | ||
| 337 | 3.1 | 1.7 | 0.1 | 4 | 0.86 |
| 338 | 3.6 | 3.4 | 4.2 | ||
| 339 | 3.4 | 3.2 | 3.2 | 4 | 425 |
| 340 | 3.8 | 2.7 | 0.1 | 4.1 | 0.18 |
| Mean | 3.1 ± 0.8 | 2.3 ± 1.3 | 2 ± 1.9 | 3.7 ± 0.7 | 142 ± 173.7 |
a
Viremia was determined after 7 (day 10) and 28 days of treatment (day 31, treatment cessation) and at the end of follow-up (day 223) by real-time PCR as described in Materials and Methods (log10 values are reported).
b
Estimation of viral production was determined by calculation of the area under the curve (AUC) during the 28 days of treatment (from day 3 to 31) and reported as log10 AUC values.
c
Quantitation of intrahepatic viral DNA in copies per vge of liver cell DNA by real-time PCR was performed on the remaining ducklings that were sacrificed at the end of the study (day 223).