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GC separation of free fatty acids (as methyl esters) in T. rotula. A, Lipase reactions were suppressed by adding boiling water to the carefully isolated intact cells before extraction. B, Cells were disrupted by sonication and extracted after 10 min. IS, Internal standard; 1, myristic acid (C14:0); 2, palmitic acid (C16:0); 3, stearic acid (C18:0); 4, unsaturated C16:n fatty acids; 5, unsaturated C18:n fatty acids; 6, eicosapentaenoic acid (C20:5).
