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. 2002 May;129(1):103–111. doi: 10.1104/pp.010974

Figure 3.

Figure 3

Left, Activity of phospholipase A2 (PLA2) in seawater medium after cell disruption. Thin-layer chromatography (TLC) separation of phospholipids and lysolipids after administration to damaged and intact T. rotula (silica; MeOH/CHCl3/HOAc; A, BODIPY-3-pentanoic acid; B, 2-(BODIPY-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine). Lines 1 through 4, Labeled phospholipid treated with broken cells of T. rotula after 1, 3, 8, and 20 min; line 5 through 7, control treatments of intact cells after 1, 8, and 20 min show no PLA2 activity in the medium; line 8, labeled phospholipid; line 9, labeled phospholipid treated with bee venom (Sigma, Deisenhofen, Germany) phospholipase A2. Right, Test for triacylglycerol lipase activity [silica; ether; C, glycerol tris-(1-pyrenebutyrate)]. Line 10, Labeled tris-acylglycerol treated for 20 min with broken cells of T. rotula, no lysolipids, and labeled fatty acids with lower ratio of fronts compared with glycerol tris-(1-pyrenebutyrate) are detected; line 11, labeled lipid.