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. 2002 May;129(1):156–168. doi: 10.1104/pp.000869

Figure 2.

Figure 2

Northern- and Southern-blot analyses. A, DNA gel-blot analysis. Ten micrograms of tomato genomic DNA was restricted with XbaI (1), HindIII (2), EcoRI (3), and DraI (4), and was separated by agarose gel electrophoresis. DNA fragments were transferred to nitrocellulose membranes and the blot was hybridized with the radiolabeled 3′-untranslated region of the LeCPK1 cDNA. The position of DNA standards (1-kb ladder, Life Technologies/Gibco-BRL, Cleveland) is indicated and their size is given in kb. B, RNA gel-blot analyses. Five micrograms of total RNA isolated from the leaves of control plants (lane 1) and plants at 0.5, 1, 1.5, 2, 4, 6, or 8 h (lanes 2–8) after treatment with 3 μm FC was separated on formaldehyde agarose gels and subsequently transferred to nitrocellulose membranes. The blots were hybridized with the radiolabeled 3′-untranslated region of the LeCPK1 cDNA and the cDNAs of 14-3-3 and pathogenesis-related proteins, as indicated. A duplicate gel was stained with ethidium bromide as a control of RNA quantity and integrity.