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. 2002 May;129(1):211–224. doi: 10.1104/pp.010690

Figure 4.

Figure 4

E. coli carrying 11 nod genes can trigger calcium spiking in M. truncatula. A, Root hair deformation response to DKR61 (carrying common and host-specific nod genes), DKR64 (carrying host-specific nod genes), DKR63 (carrying common nod genes), and BL21 (DE3). Black arrowhead marks a branched root hair. B, Calcium spiking response of three representative root hairs to DKR61 [BL21 (DE3) pRmE2 pRmJT5]. C, Calcium spiking response to DKR63 [BL21 (DE3) pRmE2]. D, Calcium spiking response to DKR64 [BL21 (DE3) pRmJT5]. E, Calcium spiking response to E. coli BL21 (DE3). Root hair deformation (A) and calcium spiking phenotypes (B–E) elicited by E. coli BL21 (DE3) strains carrying common and/or host-specific nod genes. Root hair deformation assays were scored 48 h after inoculation with bacteria, as described in “Materials and Methods.” In B through E, E. coli cells were added at time = 0 min, marked by the solid vertical line, and NF and/or Rm1021 cells were added subsequently as a positive control.