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R. leguminosarum bv viciae triggers calcium spiking in vetch and M. truncatula but not alfalfa. Representative traces of calcium response elicited by R. leguminosarum bv viciae strain A34 in vetch (A), M. truncatula (B), and alfalfa (C). Bacteria were added at time = 0 min in all cases. For alfalfa, where A34 cells failed to induce a response, purified NF was added back to verify the root hair's ability to initiate calcium spiking.
