Table III.
Strain or Plasmid | Relevant Characteristics | Source or Reference |
---|---|---|
S. meliloti | ||
Rm1021 | Wild type, SU47 str21 | Meade et al. (1982) |
SL44 | Rm1021 ΔnodD1ABC | Fisher et al. (1988) |
GMI3253 | Rm1021 ΔnodA | Debellé et al. (1996) |
JAS108 | Rm1021 nodF∷Tn5-233, Spr a | J.A. Swanson (unpublished data) |
JAS115 | Rm1021 nodE∷Tn5-233, Spr | J.A. Swanson (unpublished data) |
GMI6436 | Rm2011 nodL∷Tn5, Nmr b | Ardourel et al. (1995) |
RJW13 | Rm1021 nodL∷Tn5, Nmr | This study |
JT210 | Rm1021 nodH∷Tn5, Nmr | Swanson et al. (1987) |
912T | Rm1021 nodH∷Tn5, Nmr | J. Ogawa (unpublished data) |
RJW25 | 912T pRmE65 | This study |
GMI3032 | Rm2011 ΔnodF nodL∷Tn5, Nmr | Ardourel et al. (1994) |
RJW14 | Rm1021 ΔnodF nodL∷Tn5, Nmr | This study |
R. leguminosarum bv viciae | ||
A34 | Wild type, 8401 pRL1JI | Downie et al. (1983) |
RJW1 | A34 pJT5 | This study |
RJW18 | A34 pRmS210 | This study |
E. coli | ||
BL21 DE3 | F−ompT, hsdSR (rB−mB−) gal dcm (DE3) | Studier and Moffatt (1986) |
DKR 61 | BL21 DE3 pRmE2 pRmJT5 | This study |
DKR 63 | BL21 DE3 pRmE2 | This study |
DKR 64 | BL21 DE3 pRmJT5 | This study |
Plasmids | ||
pRmE65 | nodD3 Expressed under control of trp promoter in pTE3 | Fisher et al. (1988) |
pRmJT5 | nodFEGHPQ1 syrM nodD3 in pLAFR1 | Swanson et al. (1987) |
pRmS210 | nodH∷Tn5 in pJT5 | Swanson et al. (1987) |
pRmE2 | nodABC in pAD10 | Egelhoff and Long (1985) |
pRK600 | Helper plasmid derived from pRK290 | Glazebrook and Walker (1991) |
Phage | ||
N3 | S. meliloti-transducing phage | Martin and Long (1984) |
Spr, Spectinomycin resistant.
Nmr, Neomycin resistant.