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. 2002 May;129(1):257–268. doi: 10.1104/pp.010957

Figure 3.

Figure 3

A, GC-MS analyses of the products formed in the incubation of a 20,000g supernatant from chicory roots with NADPH and germacra-1(10),4,11(13)-trien-12-oic acid (14) displays peaks of dehydrosaussurea lactone (dHSausL [16]), saussurea lactone (SausL [18]), and leucodin (Leuc [19]). B, These products are not observed in the absence of NADPH. Germacrene-1(10),4,11(13)-trien-12-oic acid (14) is observed as elema-1,3,11(13)-trien-12-oic acid (EAc) plus its diastereomer (EAc*); the internal standard (i.s.) is 1 nmol of cis-nerolidol. The huge fronting peaks (Inline graphic) are fatty acids (palmitic and linoleic acid). C, The standard of 0.5 mm costunolide (15) in ethanol yields a tailing peak of dehydrosaussurea lactone (dHSausL [16]).