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Requirements for (+)-costunolide synthase activity
| Addition | Percentage Product Formation ± sd |
|---|---|
| None | 0 ± 0 |
| 1 mm NADPH | 100 ± 8a |
| 1 mm NADH | 7 ± 2 |
| 1 mm NADPH + 1 mm NADH | 109 ± 23 |
| 1 mm NADP++ NAD+ | 1 ± 1 |
| 1 mm NADPH + argon atmosphere | 0 ± 0 |
One hundred percent enzyme activity corresponds to the summation of a dehydrosaussurea lactone (16), saussurea lactone (18), and leucodin (19) peak size of 0.35, 0.19, and 0.09 × internal standard (1 nmol of cis-nerolidol), respectively.
