Figure 1.

Northern analysis of PKABA1 transcript levels in barley aleurone treated with GA and ABA. For mRNA analysis, barley embryoless half-seeds were imbibed in water and incubated for 4 days. Then, the aleurone layers were isolated and treated for 24 h with control buffer (20 mM succinate, pH 5, containing 10 mM CaCl₂) plus: GA (2 μM GA₃); GA + ABA (2 μM GA₃ + 20 μM ABA); GA + 8′-acet-ABA (2 μM GA₃ + 20 μM 8′-acetylene-ABA). Total RNA was extracted and Northern analysis was conducted with the PKABA1 cDNA clone. Ethidium bromide-stained 28S and 18S rRNA are shown as controls for loaded RNA amounts. Northern analysis results are representative of three separate experiments assessing PKABA1 mRNA levels in aleurone layers treated for 24 h with ABA and control buffers.