Figure 3.

PKABA1 suppresses the GA₃-induced expression of a cysteine proteinase gene. (A) Schematic diagram of the effector and reporter constructs used in cobombardment experiments. (B) The reporter construct, EPB1-GUS, and the internal control construct, UBI-luciferase, were cobombarded into barley half-seeds either with or without the effector construct (UBI-PKABA1) using the same amount of effector and reporter constructs (1.14 μg per shot). Transfected barley half-seeds were incubated for 24 h with or without 1 μM GA₃ and/or 20 μM ABA. GUS activity was normalized in every independent transformation relative to the luciferase activity. Data are means ± SE of four replicates in a typical experiment. The experiment was repeated three times, and similar results were obtained.