Figure 4.

PKABA1 has a small effect on the response of ABRC3 (ABA response complex 3) to ABA treatment. (A) Schematic diagram of the effector and reporter constructs used in cobombardment experiments. (B) The effector construct, UBI-PKABA1, was cobombarded into barley half-seeds along with the reporter construct (ABRC3-GUS) and the internal control construct (UBI-luciferase). The amount of reporter and control plasmid DNA was always constant (1.14 μg per shot), whereas that of effector varied with respect to the reporter as shown in the x axis. Transfected barley half-seeds were incubated for 24 h with (■) or without (●) 20 μM ABA. GUS activity was normalized in every independent transformation relative to the luciferase activity. Data are means ± SE of four replicates in a typical experiment. The experiment was repeated three times, and similar results were obtained.