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. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: Nat Methods. 2005 Aug;2(8):599–605. doi: 10.1038/nmeth777

Figure 5.

Figure 5

Axons can be cocultured with other cell types such as oligodendrocytes. Oligodendrocytes were added to the axonal compartment of 7 d in vitro neurons. (a) Low-power confocal image demonstrates the coculture of neurons (red, neurofilament) with oligodendrocytes (green, myelin basic protein) in separate compartments for eight additional days. In some cases, oligodendrocytes align themselves along axons (arrows) in a pattern reminiscent of white matter tracts in vivo. Dashed lines indicate the barrier region. Scale bar, 60 μm. (b) Higher-power views demonstrate the association of oligodendrocyte processes (green) with neuronal axons (red). Scale bars, 30 μm(left) and 10 μm (right). (c) A single confocal optical Z-slice demonstrates myelin basic protein immunoreactive processes surround an axon. Scale bar, 4 μm.