Figure 5.

PTYR fractionation. AGS cells were infected with the Hp wild-type strain G27 or its isogenic mutant Δ527 as described (12). Host cell cytosol and membrane proteins were separated from the cellular fraction containing the bacteria, unlysed cells, and the cytoskeleton proteins. The fractions were analyzed by SDS/8% PAGE and transferred onto four separate nitrocellulose membranes. Each membrane was probed with a different antibody [anti-PY (A), LDS 56 (B), anti-urease (rabbit polyclonal antiserum against recombinant UreA; C), or anti-HGFR (C28, Santa Cruz Biotechnology; D)]. Estimated mobility is provided for the phosphorylated (A; 138 kDa) and unphosphorylated CagA (B; 136 kDa), urease A (C; 58 kDa), and hepatocyte growth factor receptor (HGFR) major subunit (D; 145 kDa).