Figure 4. Effects of detergent on protease cleavage.

fXa protease cleavage of Lag1–fXa and Lac1–fXa fusion proteins with various detergent treatments. The microsomes from the transformants used in Figure 3 were prepared and mock-digested or digested with fXa protease in the absence or presence of 1% NP40, 1% digitonin, 1% Triton X-100 and 1% SDS on ice for 24 h, and resolved on 13.5% polyacrylamide gels. The Lag1 and Lac1 proteins were detected by immunoblotting using anti-FLAG antibodies.