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. 2006 Aug 29;398(Pt 3):509–514. doi: 10.1042/BJ20060566

Figure 2. Representative RP-HPLC analysis of plasma from wild-type mice.

Figure 2

Wild-type pups received hypodermic cysteine and the plasma concentration of NAC and cysteine was measured at 0 h (upper left panel), 2 h (upper right panel), 4 h (lower left panel) and 8 h (lower right panel) from injection. For this purpose, the plasma was derivatized with nitrous acid and S-nitroso derivatives of 2-mercaptoethanol as internal standards (IS); NAC and cysteine (Cys) were separated by RP-HPLC. Note that at 0 and 8 h from injection, NAC is present in trace amount.