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. 2006 Aug 29;398(Pt 3):431–437. doi: 10.1042/BJ20060380

Figure 2. Complex-associated forms of Id2 in the nuclei of quiescent liver.

Figure 2

Nuclear extracts were immunoprecipitated with antibodies against p130 (α-p130), Id2 (α-Id2), mSin3A (α-sin3A) and E2F4 (α-E2F4). The proteins in the immunoprecipitates were subjected to electrophoresis (8% PAGE for p130 and 15% PAGE for Id2) and analysed by Western blotting using the antibodies against p130 (B, upper panel) and Id2 (B, lower panel). To assess the specificity of p130 and Id2 antibodies, the input sample (5% of total nuclear aliquot) was also analysed by Western blotting (A). Molecular masses (MW) are given in kDa. As a control, a sample was treated in the immunoprecipitation protocol with a non-related antibody (NR Ab). A further control was carried out in the experiment without added antibody (No Ab).