Figure 6. mSin3A and Id2 are released in vivo from the c-myc promoter in cirrhotic rats when the gene is being transcribed.

The experiment was conducted as in Figure 3, with samples from rat cirrhotic liver at 28 days after BDL. (A) ChIP analysis was carried out with the antibodies indicated over the PCR results in control and cirrhotic rats. The primers were specific for c-myc promoter. (B) Real-time RNA pol-ChIP analysis of c-myc transcription in normal and cirrhotic rats. An antibody against RNA pol II (α-RNA pol II) was used for immunoprecipitation of chromatin, and the bound DNA was analysed with primers from the third exon of the c-myc gene. No Ab, no antibody.