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. 2006 Aug 29;398(Pt 3):399–409. doi: 10.1042/BJ20060033

Figure 2. Phosphatase activity of hVps29 on a CI-M6PR phosphopeptide.

Figure 2

(A) Coomassie Blue staining of recombinantly expressed GST fusion proteins of vector (26 kDa), hVps26 (36 kDa), hVps29 (20 kDa) and hVps35 (87 kDa) before and after thrombin cleavage. (B) Detected amounts of free phosphate in an in vitro phosphatase assay on the SFHDDpSDEDLLHI phosphopeptide using recombinantly expressed vector, hVps26, hVps29 or hVps35 or a mixture of these three proteins. Levels of free phosphate were determined after 30 min and after 16 h of incubation at 37 °C. (C) Coomassie Blue staining of the remaining supernatant of the protein samples used for the in vitro phosphatase assay.