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. 2006 Aug 29;398(Pt 3):547–556. doi: 10.1042/BJ20060299

Figure 7. S1P-evoked ML-1 cell migration is inhibited by the S1P1/3 antagonist VPC 23019 and the PI3K-inhibitor wortmannin.

Figure 7

(A) Migration towards the indicated concentrations of S1P in control cells (white bars) or cells pre-treated with 1 μM VPC 23019 for 1 h (hatched bars). The S1P1-specific agonist SEW-2871 (1 μM; black bar) does not induce migration. n=3. (B) Migration towards S1P in control cells (white bars) or cells pre-treated with VPC 23019 (hatched bars) in the presence of 10% (v/v) FCS. ◆ indicates statistically significant difference compared with VPC 23019-treated control cells. Results are means±S.E.M., n=3. (C) Migration towards S1P in control cells (white bars) or cells pre-treated with 100 ng/ml wortmannin for 1 h (black bars). Results are means±S.E.M., n=4. (D) Migration towards S1P in control cells (white bars) or cells pre-treated with wortmannin (black bars) in the presence of 10% (v/v) FCS. * indicates statistical significance as indicated by parentheses. ◆ indicates statistically significant difference compared with wortmannin-treated control cells. Results are means±S.E.M., n=4. */◆P<0.05; **P<0.01; ***/◆◆◆P<0.001 (one-way ANOVA and Holm's post hoc test).