Figure 6. Expression of PDA synthase.

(A) Poly(A)⁺ RNA (2.5 μg) extracted from wild-type Drosophila (Oregon-R strain) at different developmental stages was analysed by Northern blotting with a ³²P-labelled cDNA probe covering the full-length CG6781 ORF. Developmental stages were: 1, early embryos (0–9 h); 2, late embryos (9–18 h), 3, second instar larvae; 4, third instar larvae; 5, white pupae; 6, brown pupae; 7, 0–2-day-old adults; 8, 3–4-day old adults; 9, 5–6-day-old adults. (B) Total RNA (90 μg) extracted from 0–2-day-old adult fly heads (lane 1), and decapitated bodies (lane 2), was analysed by Northern blotting. The lower panel in (A, B) shows the results of hybridization of the same blot with a ³²P-labelled rp49 cDNA probe.