Table 2. Enzyme activity of recombinant candidate CG products with various substrates.

The activity for each substrate was determined under standard conditions as described in the Experimental section. All values are the means for three determinations. ND, not detectable.

	Specific activity (units/mg)
	CG6781	CG6673A	CG6673B	CG6776	CG6662
PDA synthase*	124	ND	ND	ND	ND
GST activity for CDNB†	ND	0.01	0.08	ND	ND
Thiol transferase‡	5.62	2.83	54.26	1.06	0.55
DHA reductase§	3.00	74.34	133.29	0.71	0.26

*One unit of enzymatic activity is defined as the amount of enzyme needed for the formation of 1 nmol of PDA from H₂NTP in 2 h at 30 °C in the presence of excess PTP synthase.

†One unit of enzymatic activity is defined as the amount of enzyme catalysing the displacement of 1 μmol of chloride per min at 25 °C.

‡One unit of enzymatic activity is defined as the amount of enzyme catalysing the formation of 1 μmol of GSSG per min at 30 °C.

§One unit of enzymatic activity is defined as the amount of enzyme catalysing the formation of 1 μmol of DHA per min at 30 °C.