Table 1. Parameters for Hfra thermal denaturation as a function of pH.
pH | Tm (°C) | ΔHm (kJ·mol−1) | ΔTm (°C) | Refolding (%) |
---|---|---|---|---|
4.9 | 59.0±0.1 | 275.1±1.7 | −7.3±0.1 | 20 |
6.0 | 65.4±0.2 | 371.8±2.1 | −0.9±0.3 | 80 |
7.0 | 62.5±0.2 | 250.4±1.3 | −3.8±0.3 | 95 |
7.9 | 66.3±0.1 | 373.5±1.7 | – | 100 |
8.9 | 62.1±0.2 | 362.2±3.3 | −4.2±0.3 | 100 |
9.7 | 59.0±0.1 | 282.2±1.3 | −7.3±0.1 | 20 |