Table 2. Parameters for the chemical denaturation of wild-type hfra, pH 7.9.
| Denaturant | [D]1/2 (M) | m (kJ·mol−1·M−1) | ΔGH2O (kJ·mol−1) |
|---|---|---|---|
| GuSCN | 1.1 | 22.4±1.4 | 24.6±1.7 |
| GdmCl | 2.1 | 16.8±1.4 | 35.3±2.1 |
| Urea | 4.3 | 5.7±0.2 | 24.5±1.3 |
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