Table 3. Effect of FRDA mutations on quencher accessibility and Stern–Volmer constants using different quenchers.
KSV (M−1) | ||
---|---|---|
Protein | Acrylamide | KI |
Wild-type | 11.2±0.2 | 5.0±0.2 |
W155R | 4.5±0.1 | 3.2±0.1 |
I154F | 10.5±0.2 | 5.9±0.2 |
KSV (M−1) | ||
---|---|---|
Protein | Acrylamide | KI |
Wild-type | 11.2±0.2 | 5.0±0.2 |
W155R | 4.5±0.1 | 3.2±0.1 |
I154F | 10.5±0.2 | 5.9±0.2 |