Figure 3. Increase in Mn transport due to induction of DMT1 expression.

⁵⁴Mn²⁺ uptake was compared in the inducible cell lines with doxycycline (2/−dox and 1A/+dox) or without (2/−no and 1A/+no) and in the cell lines containing the empty vector or the tetres element only. Uptake is plotted logarithmically to allow the range of response to be shown. This comparison is representative of four repetitions. Although doxycycline might increase or decrease incorporation in cell lines containing empty vector or the tetres element only, transport was unaffected (0.10<P<0.95 for six independent comparisons).