Figure 4. Effect of decavanadate on the RNA binding activity.

(A) Increasing amounts of RNA were added to a 480 nM solution of the enzyme in binding buffer [50 mM Tris/HCl and 50 mM KOAc, (pH 7.5)] and the emission spectrum was scanned from 310 to 440 nm. (B) A saturation isotherm was generated by plotting the change in fluorescence intensity at 333 nm as a function of added RNA. The RNA binding assays were performed in the absence (■) or presence of 1 mM decavanadate (○).