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. 2006 Aug 29;398(Pt 3):485–495. doi: 10.1042/BJ20051620

Figure 1. Water permeability of POPC liposomes with varying amounts of chol and SM.

Figure 1

Liposomes were prepared with 2 mM entrapped CF and rapidly exposed to a doubling of external osmolality. (A) Effect of increasing chol on water permeation kinetics. Stopped-flow tracings (average of eight to ten trials) show quenching of CF as vesicles shrink. Fluorescence was converted into relative volume and single exponential curves were fitted to the data. The residuals from those fits are shown for each composition below (A, B). (B) Effect of increasing SM on water permeation kinetics. The inset shows more detail of the curves for 0 and 10 mole% SM. (C) Osmotic water permeability of liposomes with varying chol concentrations. (D) Osmotic water permeability of liposomes with varying SM concentrations. Results in (C, D) are from three to five different liposomal preparations. Results shown are the means±S.E.M.