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. 2006 Aug 29;398(Pt 3):485–495. doi: 10.1042/BJ20051620

Figure 2. Proton permeability of POPC liposomes with varying amounts of chol and SM.

Figure 2

Liposomes were prepared with 2 mM entrapped CF and rapidly exposed to a pH gradient of 0.3–0.5 pH unit. (A) Effect of increasing chol on proton permeation kinetics. Stopped-flow tracings (average of eight to ten trials) show quenching of CF as intravesicular pH decreases. Fluorescence was converted into pH and single exponential curves were fitted to the data. The residuals from those fits are shown for each composition below (A, B). (B) Effect of increasing SM on proton permeation kinetics. (C) Proton permeability of liposomes with varying chol concentrations. (D) Proton permeability of liposomes with varying SM concentrations. Results in (C, D) are from three to five different liposomal preparations. Results shown are the means±S.E.M.