Figure 3. .

The network-forming abilities of the wild-type and R416W GFAP in SW13/cl.1 cells. SW13/cl.1 cells transiently transfected with either wild-type (A and B) or R416W (C and D) GFAP were fixed at 48 h after transfection and were processed for double-label confocal immunofluorescence microscopy. GFAP immunofluoresence is shown in the green channel (A and C), whereas the counterstaining for vimentin is in the red channel (B and D). Notice that wild-type GFAP (A) incorporated into endogenous vimentin (Vim) (B) networks, whereas this is not the case for R416W GFAP (C). Whereas some transfected cells exhibited one large inclusion with small aggregates at the cell periphery (arrowheads in C), other transfected cells displayed bundled filaments (arrows in C) that coaligned with the endogenous vimentin (arrows in D). Bars = 10 μm.