Table 1. .

Novel Microsatellite Markers^[Note]

	Oligonucleotide(5′→3′)
Markera	Sense	Antisense	Size Range (bp)	Heterozygosity
DXYS10136	CTGAACTCAGAATCGGGACC	CCCAGGAGCCCAGGAGATTGA	303–323	.85
DXYS10137	CCCAGGCCCTGTTTACGCTTCG	TATCCTCACAACTGCGTCTTCC	180–210	.90
DXYS10138	GTACATAGATGGCAGATAGATG	CTGCATGTATACACACTGTAAT	197–221	.73
DXYS10139	AGCCCCAACCCTCCATGATACTGA	GCAAAGGCATCTGTTTAAGTAACG	131–203	.24

Note.— PCR conditions for the amplification of microsatellites DXYS10136, DXYS10137, DXYS10138, and DXYS10139 were as follows: 1× Qiagen Hotstart Taq buffer, 0.5 units of Hotstart Taq polymerase, 2.0 mM MgCl₂, 200 μM dNTPs (50 μM each), and 400 nM of each primer. Cycling conditions for DXYS10137, DXYS10138, and DXYS10139 were as follows: initial denaturation at 94°C for 15 min; 35 cycles for 30 s at 94°C; 30 s at 49°C, 58°C, and 55°C, respectively; and 40 s at 72°C, with a final extension for 8 min at 72°C. For the amplification of DXYS10136, we used a touchdown protocol for easier allele calling. Cycling conditions were as follows: initial denaturation at 94°C for 15 min, 16 cycles for 30 s at 94°C, 30 s at 58°C–66°C ramp (−0.5°C per cycle), and 40 s at 72°C, followed by 16 cycles for 30 s at 94°C, 30 s at 58°C, and 40 s at 72°C. The program terminated with a final extension for 8 min at 72°C.

^aFurther details can be found at the GDB Human Genome Database.