Figure 4. .

Antibody design for REEP1. The proteins REEP1 through REEP4 are fairly conserved in human; thus, we chose two peptides in the less-conserved C terminal of REEP1 to be targeted by two polyclonal antibodies (frames). Two rabbits were injected with the synthetic peptides. Blood was collected after 50 d and was purified through keyhole-limpet-hemocyanin columns. All peptide synthesis work and antibody production was performed at Bethyl Laboratories.