Figure  5. .

In situ hybridization of human retina probed with antisense and sense KCNV2 riboprobes. Sections 10 μm thick, from fixed and cryoprotected human retinal tissue, were probed with either antisense or sense digoxigenin (DIG)–labeled riboprobes generated from a 427-bp gene fragment, cloned into pGemT Easy vector, that spans the central portion of exon 1. Retinal sections were prepared for hybridization with the use of standard methods. Hybridization and washing was performed at 65°C. Signal was resolved using anti-DIG antibody at a 1:2,000 dilution, followed by color development. OS = Photoreceptor outer segment; IS = photoreceptor inner segment; ONL = outer nuclear layer.