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. 2006 Jul 24;79(3):574–579. doi: 10.1086/507568

Table 1. .

PCR Primer Pairs for PDE6H, KCNV2, KCNB1, KCNC1, and KCNF1

Primer(5′→3′)
Gene and
Primer Pair
Exon Size
(bp)
Forward Reverse
PDE6H:
 A 1 290 GGTCCCCATGACTTGAAAGATC AAACAAAAGCAGGTTTTGTGGG
 B 2 159 TCTCCATGTGAGTGACTCCAA GCAGAACTCCAAGTGCGAAGT
 C 3 328 AGGAGTGAAGTGTCTCTGCCT CAGTAAGAGAACTCTTAGTAG
KCNV2:
 A 1 1,133 TAGAGGCAGTGAGCAGGTGA CACTCGAGCAGCAGCTGA
 B 1 826 TAGAGGCAGTGAGCAGGTGA TCTCCATGAGGTTCCAGAGG
 C 1 1,184 GCTGGACTACTGCGAGCTG ATCCATCGCCGTTTTGTTAG
 D 1 822 AGGAACTCTTCCGCGACAT ATCCATCGCCGTTTTGTTAG
 E 2 448 GCTTGCTCCTCTCCCTTTCT CATTTCCTTTTGCTGCCAAT
 F 1 731 GCTGGACTACTGCGAGCTG CACTCGAGCAGCAGCTGA
 Ga 1 427 GCTGGACTACTGCGAGCTG TCTCCATGAGGTTCCAGAGG
KCNB1:
 H 424 GGGAGCGGGAAGGCGAGGAGTT CTGGACCACGCGGCGGACATTC
KCNC1:
 I 566 TGGCCTTCGCTTCCCTCTTCTTCA GCTCACTGGCGCTGGGGTCATTG
KCNF1:
 J 536 TGCTGGCCATCCTCCCCTTCTACG CGCCCCCGCTGCTGGAGTTGAG
a

Primer pair G was used for the amplification of a KCNV2 gene fragment, for the generation of riboprobes for in situ hybridization.