Table 1. .
Primer(5′→3′) |
||||
Gene and Primer Pair |
Exon | Size (bp) |
Forward | Reverse |
PDE6H: | ||||
A | 1 | 290 | GGTCCCCATGACTTGAAAGATC | AAACAAAAGCAGGTTTTGTGGG |
B | 2 | 159 | TCTCCATGTGAGTGACTCCAA | GCAGAACTCCAAGTGCGAAGT |
C | 3 | 328 | AGGAGTGAAGTGTCTCTGCCT | CAGTAAGAGAACTCTTAGTAG |
KCNV2: | ||||
A | 1 | 1,133 | TAGAGGCAGTGAGCAGGTGA | CACTCGAGCAGCAGCTGA |
B | 1 | 826 | TAGAGGCAGTGAGCAGGTGA | TCTCCATGAGGTTCCAGAGG |
C | 1 | 1,184 | GCTGGACTACTGCGAGCTG | ATCCATCGCCGTTTTGTTAG |
D | 1 | 822 | AGGAACTCTTCCGCGACAT | ATCCATCGCCGTTTTGTTAG |
E | 2 | 448 | GCTTGCTCCTCTCCCTTTCT | CATTTCCTTTTGCTGCCAAT |
F | 1 | 731 | GCTGGACTACTGCGAGCTG | CACTCGAGCAGCAGCTGA |
Ga | 1 | 427 | GCTGGACTACTGCGAGCTG | TCTCCATGAGGTTCCAGAGG |
KCNB1: | ||||
H | … | 424 | GGGAGCGGGAAGGCGAGGAGTT | CTGGACCACGCGGCGGACATTC |
KCNC1: | ||||
I | … | 566 | TGGCCTTCGCTTCCCTCTTCTTCA | GCTCACTGGCGCTGGGGTCATTG |
KCNF1: | ||||
J | … | 536 | TGCTGGCCATCCTCCCCTTCTACG | CGCCCCCGCTGCTGGAGTTGAG |
Primer pair G was used for the amplification of a KCNV2 gene fragment, for the generation of riboprobes for in situ hybridization.