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Immunostained and FISH-labeled human pachytene spermatocyte (microspread preparation). SCP3 is shown in red, MLH1 in green, and CREST in blue. In addition, the 11q23 probe is shown in gold, and the 22q11 probe is shown in magenta. The entire nucleus is stained with DAPI (cyan). MLH1 foci mark crossover sites.
