Table 1.
Analysis of the morphological defects and mitotic progression of mitosis in live images of RNAi transfected cells vs. antibody-injected cells.
| Measurement | Control RNAi | MCAK RNAi | Control IgG | Anti-MCAK |
| Alignment Defects | % cells (n) | % cells (n) | % cells (n) | % cells (n) |
| Chromosomes lingering at poles | 26 (17) | 51 (15) | 18 (17) | 38 (16) |
| Multiple attempts to congress | 33 (17) | 49 (15) | 18 (17) | 56 (16) |
| Not all chromosomes congress | 4 (16) | 13 (13) | 6 (17) | 27 (16) |
| Loose alignment with high oscillations | 13 (16) | 89 (16) | 24 (17) | 87 (16) |
| Total % cells with alignment defects | 39 (18) | 88 (16) | 41 (17) | 88 (17) |
| Segregation Defects | % cells (n) | % cells (n) | % cells (n) | % cells (n) |
| Straggling chromosomes | 11 (17) | 53 (12) | 25 (16) | 50 (16) |
| Lagging chromosomes | 0 (17) | 6 (12) | 6 (16) | 23 (13) |
| Total % cells with segregation defects | 12 (17) | 42 (12) | 25 (16) | 50 (16) |
| Measurement | Control RNAi | MCAK RNAi | Control IgG | Anti-MCAK |
| Spindle Defects | Avg. ± SEM (n) | Avg. ± SEM (n) | Avg. ± SEM (n) | Avg. ± SEM (n) |
| Spindle length (μm) | 15.3 ± 0.5 (14) | 12.9 ± 0.5* (12) | 14.5 ± 0.6 (16) | 12.9 ± 0.4* (17) |
| Timing of Mitotic Progression | Avg. ± SEM (n) | Avg. ± SEM (n) | Avg. ± SEM (n) | Avg. ± SEM (n) |
| Time between injection and NEB (min) | NA | NA | 5.6 ± 2.2 (17) | 2.8 ± 0.8 (18) |
| Time between NEB and LCC (min) | 17.4 ± 1.3 (14) | 23.3 ± 7.0 (12) | 16.1 ± 1.7 (15) | 32.3 ± 3.4* (17) |
| Time between LCC and Ana A (min) | 13.7 ± 2.7 (13) | 12.6 ± 1.8 (11) | 16.1 ± 1.7 (14) | 17.4 ± 2.2 (18) |
| Total time between NEB and Ana A (min) | 30.1 ± 2.7 (17) | 32.6 ± 5.9 (14) | 33.3 ± 1.7 (17) | 47.7 ± 4.1* (18) |
NEB, Nuclear envelope breakdown, LCC, the time when the last chromosome begins congressing. NA, not applicable. * Represents a p-value of <0.05 and indicates a significant difference in spindle length or timing.