Table 1.

Identified ECM biofilm proteins annotated as hypothetical or uncharacterized.

KW20 gene	gi	Strain	COG #	COG	Description	Gene	Prior ID	Prior ID
HI0121	1573076	all	773	M	UDP-N-acetylmuramate-alanine ligase	MurC	y	y
HI0145	1573101	All	3010	G	Putative N-acetylmannosamine-6-phosphate epimerase	NanE	N	N
HI0148	42628803	all	3055	S	Uncharacterized BCR	-	y	y
HI0203	1573163	All	80 6	J	RimM protein, required for 16S rRNA processing	RimM	N	N
HI0227	1573192	1 4	2731	S	Uncharacterized BCR	-	y	y
HI0246	1573213	1 3 4	0	x	No related COG	-	y	y
HI0719	1573721	all	251	J	Putative translation initiation inhibitor	TdcF	y	y
HI0760	1573769	all	2924	CO	Fe-S cluster protector protein	-	n	n
HI0847	1573861	all	3085	S	Uncharacterized BCR	-	n	y
HI1034	1574067	1 3 4	1666	S	Uncharacterized BCR	-	y	y
HI1168	68057915	all	2926	S	Uncharacterized BCR	-	y	y
HI1333	1574791	All	1534	J	Predicted RNA-binding protein containing KH domain, possibly ribosomal protein	-	N	N
HI1349	1574811	All	783	L	Starvation-inducible DNA-binding protein	Dps	Y	Y
HI1427	68058243	4	0	x	No related COG	-	y	y
HI1647	1574495	1 2 4	214	H	Pyridoxine biosynthesis enzyme	SNZ1	y	y
HI1681	53732528	2	3110	S	Uncharacterized BCR	-	y	y
HI1715	1574570	All	1949	F	Oligoribonuclease (3'->5' exoribonuclease)	Orn	N	Y

Proteins are listed in order of the gene number in the Rd strain genome (HI number). gi is the NCBI gene ID number; Strain is the specific strain as determined by peptide sequence (see figure 4 legend); COG# and COG cat were assigned by COGnitor; gene description is taken from COG number entry at NCBI; Prior ID cites whether or not the protein was identified in previous proteomic analyses of the Rd strain of HI with ID1 being a previous LC-MS/MS based study (22) and ID2 being a MALDI-TOF based study (23).