Figure 1.

Reelin-like immunoreactivity in serum, adrenal, and brain extracts of various species. (A) Serum from an adult rat (loaded at 1, 2, 3, and 4 μl per lane) was Western-blotted with G10 antibody. (B) Serum from an adult human (loaded at 1, 2, and 3 μl per lane) was blotted with antibody 142. (C–E) No reelin-like immunoreactivity is detectable in serum, adrenal glands, or brain extracts of rl/rl mice. (C) Adult mice (4–6 months old). Lanes: 1, wild-type +/+ mouse; 2, blank; 3 and 4, rl/rl mice; 5, blank; 6, wild-type mouse. (D) Three-week-old mice were sacrificed and perfused with saline, and adrenal extracts were prepared. Lanes: 1 and 2, rl/+ mice; 3, blank; 4–6, rl/rl mice; 7, blank; 8, rl/+ mouse. Protein was adjusted to ≈60 μg in lanes 1–6 and 140 μg in lane 8. (E) Brain extracts of the same mice shown in D. Protein was adjusted to ≈70 μg in lanes 1–2 and ≈140 μg in lanes 3–8. Marks indicate 420-, 310-, and 160-kDa bands.