Figure 4.

Immunoblotting of adult rat adrenal and pituitary glands. Rats were perfused extensively with saline, and then glands from several animals were pooled and extracted. An equal amount of protein (50 μg) was loaded on each lane. (A) Western blotted with antireelin antibody G10. (B) Same blot reblotted with anti-DAB1 antibody. Lanes: 1, adrenal gland; 2, intermediate-posterior pituitary dissected as a unit; 3, anterior pituitary. Marks indicate the position of molecular mass standards at 207, 121, and 81 kDa.