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(A, B) Immunoprecipitation of Met in C4-2 and PC3 with and without activation by HGF. (C) Time-dependent activation of both Erk and Akt is visible in PC3 cells but not C4-2. (D, E) Rac is activated but not cdc42 upon HGF stimulation in C4-2 cells, as seen by immunoprecipitation of active GTPases.
