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. 2006 Aug 18;7:19. doi: 10.1186/1471-2172-7-19

Figure 1.

Figure 1

Differential IFN-γ response of BALB/c and C57BL/6 splenocytes to heat-killed and live B. pseudomallei stimulation. Naïve splenocytes were stimulated with heat-killed B. pseudomallei strain KHW (HK-KHW) or live B. pseudomallei strain KHW. The ratio for HK-KHW to cells is 5:1, whereas the MOI with live bacteria is 5:1. Supernatants were harvested at 24 h for determination of IFN-γ by ELISA (A). Cell viability of B. pseudomallei infected splenocytes was examined by the XTT assay at 5 h, 10 h and 24 h post-infection (B). Asterisks * represent statistical significance (p < 0.05) as determined by the Student t test. All experiments had been repeated at least 3 times.