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The effects of cytokine neutralizing antibodies on the production of IFN-γ. Splenocytes were harvested from BALB/c mice and preincubated with anti-IL-12 anti-IL-18, anti-IL-10 or their respective isotype control antibodies, followed by treatment with live B. pseudomallei strain KHW. Supernatants were harvested at 24 h for determination by ELISA. The experiment had been repeated 3 times with the same results.
