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Summary of Met-tRNAi affinities
| Protein | Kd, nM |
|---|---|
| Wild type | 9.1 ± 0.5 |
| gT34V (gT36) | 22 ± 4.1 |
| g39–43A (g41–45) | >20,000 |
| gE40A (gE42) | 380 ± 64 |
Values are shown as average ± SD of three independent experiments. The residue numbers refer to SsIF2; those in parentheses refer to PfIF2. In the g39–43A mutant, all residues in the 39EEΦ (R/K)R43 motif are mutated to alanine.
