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. 2006 Aug 17;103(35):13022–13027. doi: 10.1073/pnas.0605789103

Fig. 3.

Fig. 3.

Effect of the BMP-4 antagonist noggin on the acquisition of adipocyte characteristics by A33 cells. One day after plating replicate dishes (22,000 cells per 6-cm dish) of A33 cells, cells were exposed or not to recombinant mouse noggin 0–100 ng/ml. Noggin treatment was continued until postconfluent dishes were treated with differentiation inducers; photomicrographs were taken after 4 days and RNA harvested after 6 days. (A and B) Photomicrographs of incipient adipocytes in untreated control A33 cells (A) or A33 cells treated with 100 ng/ml noggin (B). (C) Effect of noggin on the expression of PPARγ2 mRNA.