Fig. 2.

US28 enhances proliferation through increased cell cycle progression and cyclin D1 expression. (A) Stably transfected cells were analyzed with the GUAVA Cell Cycle flow cytometer (Stamford, U.K.). After synchronization of the cells by serum starvation, cells were grown in regular medium, and US28-WT-expressing cells showed an enhanced cell cycle progression compared with mock-transfected cells. US28-R¹²⁹A expressing cells had an intermediate proliferation index in comparison with US28-WT-expressing and mock-transfected cells. (B) Western blot analysis of total cell lysates showed that US28-WT induced the up-regulation of cyclin D1 compared with mock-transfected and US28-R¹²⁹A-expressing cells. Data are mean ± SEM of representative experiments.