Fig. 3.

Cumulative in vitro proliferative output of Hoechst subsets. Hoechst subsets were plated at 10⁴ cells per cm² on col-IV in optimized AM-KGM and passaged at 10³ cells per cm² onto uncoated tissue culture plates. (a) The cumulative total cell output of SP and NSP subsets vs. Unf cells was compared up to passage 2. (b) The cell yield vs. time was monitored over four passages to reveal any differences in long-term output.