Fig. 2.

Regulation of ACP expression by ATc treatment. (A) ACP/ACPi and ΔACP/ACPi parasites were treated with ATc for 5 days and fixed and stained with antibodies to ACP and c-myc (same exposure time for all micrographs). (B) Western blot analysis of the same set of samples using ACP antibody. p, precursor; m, mature protein; MIC2, loading control. (C) ACPi parasites were grown in the presence of ATc, and samples were collected daily for 5 days and analyzed by Western blot by using the ACP antibody (native ACP serves as loading control). Note that the precursor protein disappears within a day, suggesting fast suppression of transcription, whereas the mature protein is stable and has to be diluted by growth.