Fig. 5. Summary of the distribution of different domains at the 10q25 neocentromere region. A1 designates the position of the minimal CENP-A-binding domain before TSA (Lo et al., 2001a) or following 3 days of recovery from 33 nM TSA treatment, whereas A2 denotes the position of the minimal CENP-A-binding domain after 17 h of 33 nM TSA treatment. Solid R1 and R2 boxes denote the regions of delayed replication on mardel(10) compared with normal chromosome 10 in the absence of TSA (Lo et al., 2001a). Open R1 and R2 boxes denote the same regions whose delayed replication characteristics are abolished following treatment with TSA, noting that the major domain R2 (but not R1) is reversible after removal of TSA. Regions of high AT (>62%) and LINE interspersed repeat contents and regions of depressed Alu repeats and predicted gene contents are shown (Lo et al., 2001a). NC-MiC5 represents a 0.65 Mb stable minichromosome previously constructed in our laboratory (Saffery et al., 2001).